Cyr61/CCN1 Is Regulated by Wnt/β-Catenin Signaling and Plays an Important Role in the Progression of Hepatocellular Carcinoma
Figure 5
Cyr61 promotes growth of HCC xenograft.
HCC xenograft models were established by subcutaneous injection of HepG2 cells infected with AdCyr61 or AdRFP for 36 hrs prior to injection. Two groups of SCID mice (n = 4/group) were injected with 5.0×106 cells per mouse. (A), Cyr61 levels in HepG2 cells infected with AdCyr61 and AdRFP. (B), Animals and their tumor tissues at 21 days after injection. (C), H&E staining of tumor tissues showed fibrous connective tissues hyperplasia, inflammatory cell infiltrating and tumor cells with multinuclear in xenograft tumor samples (400×), F: fibrillar connective tissue, T: tumor. Immunohistochemical detection of Ki67 in xenograft tumor samples (400×). (D), Tumor sizes were measured at a 3-day interval starting from day 6 after injection. The graph shows the tumor volume for each treatment group. *, P<0.05.