Resveratrol Prevents Endothelial Cells Injury in High-Dose Interleukin-2 Therapy against Melanoma
Figure 8
Analysis of the functional characteristics of MDSC.
Panel A: MDSC were isolated from IL-2 and IL-2+resveratrol treatment (labeled as IL-MDSC and R-MDSC respectively). LAK cells from IL-2 treated mice were isolated and incubated with MDSC for 2 h, and then used as effectors in the cytotoxicity assay against EC cell targets. Panel B: 3H-thymidine incorporation assay to measure the proliferation of the LAK cells in the panel A. Panel C: 1×106 MDSC were intravenously transferred to naive mice. VLS was then induced in the recipient mice. Panel D & E: Total RNA was extracted from the MDSC with the respective treatment. Expression of Arg1 (D) and AhR (E) were detected by QPCR. The relative expression was normalized to the endogenous 18S.