Controlled In Meso Phase Crystallization – A Method for the Structural Investigation of Membrane Proteins
Figure 1
Monoolein/water self-organization into mesophase: time course of mesophase formation.
900 nl water was added to a protein well of a crystallization micro plate coated with 132 µg monoolein (MO). The optical properties of the forming phases were examined under a polarization microscope over time. A, t = 1 min, B, t = 5 min C, t = 21 min, D, t = 22 min, E, t = 39 min Note: Arrows indicate the lower boundary of the forming isotropic meso-phase.