Mitochondrial Lysyl-tRNA Synthetase Independent Import of tRNA Lysine into Yeast Mitochondria
Figure 1
Suppression of growth defect associated with yeast MSK1 deletion by human mitochondrial lysyl-tRNA synthetase.
Strains carrying various ectopic plasmids were grown on YEPD medium over night. 2 0D600 unit cells were pelleted and suspended in 1 ml of water. The culture was serially diluted in 10-fold steps and 10 ul of each dilution was spotted onto YEPD and YEG plates. Two lanes of Δ/hmtKARS represent two spores originated from the single tetrad.