Mycobacterium tuberculosis Rv3586 (DacA) Is a Diadenylate Cyclase That Converts ATP or ADP into c-di-AMP
Figure 4
Catalytic activities of DacA with different nucleotides.
(A) Reaction of DacA with ADP, AMP, c-di-AMP or pApA. Samples were separated by HPLC. The peaks in “DacA+ADP” are labeled according to the retention time of each standard as shown in Fig. 2A. (B) Reactions catalyzed by DacA using ATP as a substrate, based on the results shown in Fig. 2 and Fig. 4A. “A” stands for adenosine, and “P” stands for phosphate. The thickness of arrows denotes priority of reaction, and the thickest arrow shows the major catalytic reaction.