GRP78 Knockdown Enhances Apoptosis via the Down-Regulation of Oxidative Stress and Akt Pathway after Epirubicin Treatment in Colon Cancer DLD-1 Cells
Figure 3
The effect of Nrf2 on epirubicin (epi) treatment.
(A) Scrambled control and GRP78 knockdown DLD-1 cells were cultured for 48 h, and nuclear Nrf2 expression was evaluated by western blotting. (B) The GRP78 knockdown DLD-1 cells were pretreated with scrambled siRNA (sc) or Nrf2 siRNA for 24 h, and nuclear Nrf2 expression was evaluated by western blotting. (C) The GRP78 knockdown DLD-1 cells were pretreated with scrambled siRNA (scramble) or Nrf2 siRNA for 24 h and treated with 500 ng/ml epirubicin (epi) for another 48 h. After treatment, the percentage of cells that were in the subG1 phase indicated by DNA damage was determined as outlined in the Materials and Methods. These experiments were performed at least 3 times, and a representative experiment is presented.