Mixed Adjuvant Formulations Reveal a New Combination That Elicit Antibody Response Comparable to Freund's Adjuvants
Figure 2
Monoclonal antibody binding to gp140SF162 in the presence of adjuvant.
The antigenicity of gp140SF162 in the presence of adjuvants was measured by mAb binding. A panel of 12 different mAbs, representing different epitope domains of the Env, was used. The signal of mAb binding to the adjuvant-formulated gp140SF162 was calculated relative to the signal measured in the unadjuvanted sample, which is assigned to have the value of 1. The data is color coded: Yellow represents no difference in mAb binding, or no change of antigenicity as compared to the unadjuvanted sample and light blue represents up to 3-fold decrease of mAb binding (or antigenicity). Both FIA and MF59 adjuvants appeared to have little effect on antigenicity, as mAb binding was very similar to the unadjuvanted sample. On the other hand, decreased mAb bindings were detected in the presence of both of the Carbopols.