Effects of Ethanol and Acetaldehyde on Tight Junction Integrity: In Vitro Study in a Three Dimensional Intestinal Epithelial Cell Culture Model
Figure 2
Transmission electron microscopy analysis of Caco-2 cells spheroids.
A junctional complex (tight junction; TJ, adherens junction; AJ, D; desmosome) between adjacent cells is evident, apically [A]. The 3-D culture resulted in a luminal space (L) with formation of microvilli (mv) at the apical side and polarization of cells located in the cortical region of spheroid is indicated by supranuclear localization of Golgi (G) apparatuses [B].