Mechanisms Underlying the Confined Diffusion of Cholera Toxin B-Subunit in Intact Cell Membranes
Figure 6
CTxB binding has little effect on the diffusion of other cell surface molecules.
COS-7 cells expressing YFP-GL-GPI or YFP-GT46, or stained with DiIC16 were labeled with 1 µM Cy5-CTxB (YFP-GL-GPI and YFP-GT46) or Alexa488-CTxB (DiIC16) for 5 minutes at room temperature and washed prior to FRAP studies. Diffusion coefficients were measured for YFP-GL-GPI, YFP-GT46, and DiIC16 in the presence and absence of 1 µM Cy5 CTxB (mean ± SD for n = 16–32 cells). FRAP data were collected at both 20°C and 37°C. ** p<0.01, Student t-test.