Fast Homozygosity Mapping and Identification of a Zebrafish ENU-Induced Mutation by Whole-Genome Sequencing
Figure 6
A: Time course analysis of zebrafish snapc4 expression by semi-quantitative RT-PCR.
A control without addition of RNA (lane -) was included as a negative control. 3 µg of total RNA extracted at each time point were loaded on a denaturating agarose gel to check the quality and the quantity of the RNA used for each RT-PCR. B: Expression profile of the snapc4 transcripts performed by WISH at 15 hpf (12S), 24 hpf, 3 dpf and 4 dpf. C: Translation-blocking snapc4 morpholino leads to growth retardation before 24 hpf. Bright-field images of 24 hpf embryos injected with 8 ng of control morpholino or of MOATG-snapc4 : 75% of the embryos injected with MOATG-snapc4 show severe growth retardation compared to the control morphant.