Activation of ERAD Pathway by Human Hepatitis B Virus Modulates Viral and Subviral Particle Production
Figure 9
EDEM1 silencing results in increased secretion of enveloped HBV.
HepG2.2.2.15 cells were transfected twice with either EDEM1 siRNA (siEDEM1) or scrambled (siCtrl) siRNA. At 3 days pt, cell medium was collected and either immunoprecipitated with a mixture of anti-S/preS1Abs (white bars) or analysed for secretion of SVPs by ELISA (grey bars). The enveloped viral particles were further quantified following DNA purification and real-time PCR. The cut-off values in ELISA varied between 0.085–0.096. Statistical analysis showing “p” values was performed using the Student's unpaired t-test.