EGF-Induced EMT and Invasiveness in Serous Borderline Ovarian Tumor Cells: A Possible Step in the Transition to Low-Grade Serous Carcinoma Cells?
Figure 6
EGF induces cadherin switch through ERK1/2 and Akt activation in SBOT3.1 cells.
A, SBOT3.1 cells were treated for 48 hr with PD98059 (20 µM) or LY294002 (20 µM) in the presence or absence of 50 ng/ml EGF. E-cadherin and N-cadherin mRNA (left panel) and protein (right panel) levels were analyzed by RT-qPCR and western blot, respectively. B, SBOT3.1 cells were treated for with PD98059 (20 µM) or LY294002 (20 µM) in the presence or absence of 50 ng/ml EGF and Snail, and the Slug, Twist and ZEB1 mRNA levels were analyzed by RT-qPCR. C, SBOT3.1 cells were treated with 50 ng/ml EGF in combination with PD98059 (20 µM) or LY294002 (20 µM). D, MPSC1 cells were treated with 50 ng/ml EGF in combination with PD98059 (20 µM) SB203580 (10 µM) or LY294002 (20 µM). After treatment, cells were seeded into un-coated (migration) and Matrigel-coated (invasion) transwell inserts. After 24 hr (migration) and 48 hr (invasion) incubation, non-invading cells were wiped from the upper side of the filter and the nuclei of invading cells were stained with Hoechst 33258. Results are expressed as the mean ± SEM of at least three independent experiments. *p<0.05 compared with Ctrl. #p<0.05 compared with EGF.