Blimp1 Activation by AP-1 in Human Lung Cancer Cells Promotes a Migratory Phenotype and Is Inhibited by the Lysyl Oxidase Propeptide
Figure 8
LOX-PP represses the migratory phenotype of lung cancer cells via inhibiting Blimp1.
(A) A549 and (B) H441 cells were transfected with 2 µg of EV or human LOX-PP cDNA. Upper panels: After 24 h, 1×105 transfected cells were subjected to migration assay. Lower panels: After 48 h, culture media was isolated and samples (50 µl of 2 ml total) subjected to immunoblotting using an anti-V5 antibody for LOX-PP. (C and D) H441 were transiently transfected with 1 µg of LOX-PP or Blimp1 DNA alone or in combination, or EV DNA (2 µg total DNA). (C) After 24 h. cells were subjected to migration assays, in triplicate, for 16 h. The average migration from two independent experiments ± SEM is presented relative to the EV (set at 1.0). (D) WCE and media were isolated. WCE samples (25 µg) were subjected to immunoblotting for Blimp1 and α-tubulin. Media samples (50 µl) were subjected to immunoblotting for LOX-PP-V5. Immunoblots from one of two independent experiments with similar results are presented.