Functional Identification of Dendritic Cells in the Teleost Model, Rainbow Trout (Oncorhynchus mykiss)
Figure 1
Culture and enrichment of tDCs.
Phase bright (A) and phase contrast (B) images of hematopoietic cultures showing non-adherent cells with dendritic morphology. Flow cytometry forward/side scatter profile of cultured cells before and after enrichment (C). Gates indicate tDC population; typical purity after enrichment was 70–80%. (D) Low magnification of Giemsa-Wright stained cytospin of enriched cells. (E) High magnification of Giemsa-Wright stained cytospin of enriched cells.