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Critical Role of Constitutive Type I Interferon Response in Bronchial Epithelial Cell to Influenza Infection

Figure 4

H3N2 replication and IFN-β release following protein synthesis inhibition with cycloheximide in Calu-3 cells and pBECs.

Cycloheximide was added to Calu-3 cells and pBECs to inhibit protein synthesis 30 min before H3N2 infection or Poly I:C treatment. (A) GAPDH was measured at 6 h after H3N2 infection and Poly I:C stimulation by western blotting. (B) Viral replication was measured by plaque assay after 48 h. (C) IFN-β release was measured by western blotting at 48 h. Western blots of Calu-3 cells and pBECs were performed separately. Results were derived from three independent experiments and are presented as mean ± standard error of the mean (SEM). * indicates significant induction from media control. § indicates a significant reduction compared to media control.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0032947.g004