Spherical Lactic Acid Bacteria Activate Plasmacytoid Dendritic Cells Immunomodulatory Function via TLR9-Dependent Crosstalk with Myeloid Dendritic Cells
Figure 4
Morphology of pDC stimulated by LABs and incorporation into pDC.
A. L. lactis JCM5805, JCM20101 and L. rhamnosus ATCC53103 were added to pDC for 48 hrs, respectively. Cells were placed onto slide grass by cytospin and stained by Diff-Quick. B. FITC-stained L. lactis JCM5805, JCM20101 and L. rhamnosus ATCC53103 were added to BM-derived pDC, and were subsequently incubated for 24 hrs. Cells were stained with PE-Cy5.5 labeled anti-B220 antibody as pDC marker, and imaged by fluorescence microscopy.