Lack of Functional Selectin Ligand Interactions Compromises Long Term Tumor Protection by CD8+ T Cells
Figure 5
Antigen presentation and proliferation.
Mean ± SD of triplicate cultures of CFSE reduction correlates to proliferation of OT.1 CD8+ T cells in response to DCs from LM or LM-OVA infected mice. Positive controls were pulsed with OVA257–264 peptide in vitro. Data are significantly different, ***(p<0.0001), relative to negative control (LM infected DCs) by two tailed t test with Welch's correction for unequal variance. Data are representative of 2 independent DC isolation experiments. (B) Representative flow cytometric profile indicating in-vitro antigen-dependent proliferation of OVA257–264-specific CD8+ T cells on different days of in vitro culture. Samples were obtained from in-vitro cultures in T-75 flasks. Data are representative of three such independent experiments conducted.