Widespread mRNA Association with Cytoskeletal Motor Proteins and Identification and Dynamics of Myosin-Associated mRNAs in S. cerevisiae
Figure 4
2D dynamics of actin patch mRNA localization.
(A) Montage of a time-lapse video of LAS17:MS2 (green) localization in a haploid, early S phase cell with actin patch accumulation at the presumptive bud indicated by FM4–64 staining (red). Scale bar is 2 µm. (B) The trajectory of the mRNA particle in panel (A) is shown on a grayscale image of the first timepoint (0 s) with black-white inversion. Time is encoded by color in the heatmap. (C) Montage of a time-lapse video of LAS17:MS2. Scale bar is 5 µm. Brightness of the latter timepoints in the montage was adjusted relative to the earliest timepoints for display purposes in order to compensate for photobleaching. (D) The trajectory of the mRNA particle from panel (C) is displayed as in panel (B). (E) Change in the diffusion coefficient of the mRNA particle in panels (C) and (D) over time. The diffusion coefficient for short time lags (τ = 4) is graphed with time encoded by color as in panel (D). D was extracted from 4 non-overlapping segments of the trajectory of length 8.992 s, or 16 timepoints. The highest estimated D value, 0.00138 µm2/s, leads to an approximate standard deviation of 5.63×10−3 µm2/s [135], [136].