Role of Chd7 in Zebrafish: A Model for CHARGE Syndrome
Figure 9
Knockdown of chd7 results in defects in CNC development.
Fli1-GFP transgenic embryos were used for MO injections to determine whether Chd7 depletion had an effect on CNC development. Embryos were injected at the 1 cell stage and examined for effects at 34–36 hpf. (A) Control-MO injected zebrafish embryos did not exhibit defects in CNC segment shape or number. Alternatively, the majority of chd7-MO-injected embryos exhibited aberrant CNC development including an improper number of CNC segments (B), as well as severely disorganized (C) or missing CNC populations. (D) Based on the t-test comparing means, there was a significant difference in CNC defects between chd7-MO and control-MO-injected zebrafish embryos. More than half of the chd7-MO-injected fish had deformed or missing CNC segments and about one third developed an improper number of segments. ** = 0.01; ***<0.001; e, eye; mb, midbrain; pa1–5, pharyngeal arches 1–5.