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Pαx6 Expression in Postmitotic Neurons Mediates the Growth of Axons in Response to SFRP1

Figure 3

Knock-down of Pax6 in the embryonic retina does not interfere with the generation of RGCs.

Frontal cryostat sections of E19.5 retinas after electroporation at E13.5 with shRNAs constructs control and targeting Pax6 and immunostained (red) for Pax6 and Caspase 3 (a) or Islet-1/2 and Brn3 (b) as indicated in the panels. Electroporated cells are visualized with GFP (green). The graphs show the quantification of the proportion of the double positive cells for the indicated marker. Note that shRNA efficiently target Pax6 without inducing cell death (a) or changes in expression of markers for postmitotic RGCs (b). Bar indicates 10 in (a) and 40 µm in (b). Data are expressed as the mean ± SD. (*) p<0.05; (**) p<0.01. Number of axons per condition >60 (n = 3). INL, inner layer; VZ, ventricular zone.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0031590.g003