Differential Regulation of Adhesion Complex Turnover by ROCK1 and ROCK2
Figure 6
Loss of either ROCK1 or ROCK2 expression results in impaired cell migration.
Confluent HaCaT-NSC (A,B), HaCaT-ROCK1-KD (C,D) and HaCaT-ROCK2-KD (E,F) monolayers were scratched with a pipette tip. Phase contrast images were taken immediately and 6 hours after wounding and representative phase-contrast images are shown (A–F). Relative wound closure was calculated for HaCaT-ROCK1-KD and HaCaT-ROCK2-KD cells (G) as well as SCC12f keratinocytes transiently transfected with siRNA directed against ROCK1 and ROCK2 (H). The means and standard errors of 3 separate experiments are shown and statistical significance calculated using Mann-Whitney test (*** p<0.001, * p<0.05).