Differential Macrophage Activation Alters the Expression Profile of NTPDase and Ecto-5′-Nucleotidase
Figure 6
The NTPDase activity on macrophages after phenotype differentiation alters the susceptibility to ATP induced cell death.
(A) After macrophages differentiation these cells were treated for 3 h with 2 mM ATP in the presence or absence of potato apyrase (apy; 2 U) or P2X7 receptor antagonists (3 µM KN-62, 10 µM A438079). The total number of the cells was counted in five random fields in visible filter and the cells positive for PI were counted in same fields but with ultra-violet filter. Data show mean±SD of three experiments. Significantly different from resident macrophages for ***p<0.001 or *p<0.05, two-way ANOVA with Tukey's post-hoc test. (B, C, D) UV and (E, F, G) visible representative images (magnification: 40×). M1 (stimulated with LPS) and M2 (stimulated with IL-4) macrophages were compared to resident macrophages.