Amyloid Precursor Protein and Proinflammatory Changes Are Regulated in Brain and Adipose Tissue in a Murine Model of High Fat Diet-Induced Obesity
Figure 8
APP, CD45, and CD68 immunoreactivity increased in the subcutaneous (abdominal) fat from high fat versus control diet fed mice with no robust change in Aβ immunoreactivity.
C57BL6/J mice at 6 weeks of age and weight matched were fed, ad libitum, a control (5.5% fat/weight) or high fat (21.2% fat/weight) diet for 22 weeks. Subcutaneous abdominal adipose tissue was collected, immersion fixed in 4% paraformaldehyde, sectioned, and immunostained using anti-APP, Aβ, CD45 and CD68 antibodies and antibody binding visualized using Vector VIP as the chromogen. Representative images from 12 animals per condition are shown.