The Chemotactic Defect in Wiskott-Aldrich Syndrome Macrophages Is Due to the Reduced Persistence of Directional Protrusions
Figure 1
WASp is required for the first wave of actin assembly in response to CSF-1.
(A) F-actin content of RAW/LR5 cells in response to CSF-1 stimulation at 37°C or 22°C was quantitatively measured and normalized to the cell number, as described in Material and Methods. (B) F-actin content of RAW/LR5 (LR5), shControl (Ctrl) and shWASp cells in response to CSF-1 stimulation at 22°C was measured as in (A). n = 3–4 experiments per condition. (C) Single cell analysis of F-actin content of shControl, shWASp and Y291F cells in response to CSF-1 stimulation at 22°C. n = 3 experiments, minimum 30 cells were analyzed per condition. (D) Ruffling index of shControl, shWASp and Y291F cells in response to CSF-1 stimulation at 22°C. n = 3 experiments, minimum 30 cells were analyzed per condition. Error bars represent SEM. * p<0.05 compared to unstimulated condition.