MicroRNA-211 Expression Promotes Colorectal Cancer Cell Growth In Vitro and In Vivo by Targeting Tumor Suppressor CHD5
Figure 1
Comparison of CHD5 and miR-211 expression in various colorectal cancer cell lines.
(A) RNA sequence map of the 3′-UTR of CHD5 (Gene ID 26038) mRNA with the complementary site (3′-UTR 130–136) for the seed region of miRNA-211. (B) miR-211 levels in RKO, HCT-116, RKO-S, and RKO-AS cell lines by quantitative RT-PCR based on miR-211/U6 expression fold values. (C) CHD5 protein levels in two colon cancer cell lines (RKO and HCT-116) and two established cell lines (RKO-S and RKO-AS) with enforced CHD5-S expression were analyzed by Western blot and semi-quantified based on CHD5/β-actin relative intensities. Bio-Rad Quantity One software was used for densitometric analysis of the Western blots. (D) Comparison of CHD5 and miR-211 expression levels in HCT-116 and RKO cell lines. * indicated as P<0.05.