Roles of Candida albicans Gat2, a GATA-Type Zinc Finger Transcription Factor, in Biofilm Formation, Filamentous Growth and Virulence
Figure 2
Role of Gat2 in biofilm formation.
The parent strain for the gat2/gat2 mutant and WT was SN152 (MTLa/α) [18]. A. Biofilm formation on the plastic bottoms of the strains (WT+ vector, WT+TETp-GAT2, gat2/gat2 mutant+vector, gat2/gat2 mutant+TETp-GAT2) at 30°C. Overnight cultures were inoculated into a 96-well plate and then incubated for 48 hours with shaking. The plates were washed with water and imaged. The numbers of cells adhered to the bottom represented three independent experiments. B. Biofilm formation on the plastic bottoms at 37°C. The same assay was used as in Panel A. C. Biofilm formation on silicone rubbers at 30°C. The silicone was incubated in a 24-well plate. The number of cells adhered to the silicone represented three independent experiments. D. Biofilm formation on silicone rubbers at 37°C. The number of cells adhered to the silicone represented three independent experiments.