Bmp7 Functions via a Polarity Mechanism to Promote Cloacal Septation
Figure 2
Activity of the Bmp/Smad and JNK pathways in the murine cloacal region.
Wild-type and Bmp7-/- sagittal sections at E12.5 were immunolabeled for pSmad1/5/8 (A, F) and phospho-c-Jun (pJun) (B,G). Portions of sections labeled with pJun (B, G) are shown at high resolution: (C) wild type rectum, (D) wild type urethra, (G) Bmp7 -/- rectum, and (I) Bmp7 -/- urethra. Nuclei are labeled with DAPI. Scale bars: 100 µm. (E) Graphic presentation of the density of pJun+ cells in the cloacal endoderm (CE) and URM in wild-type (red bars) and mutant cloacas (blue bars). Student's t-test: *P<0.01; ** P<0.005. (J) Western analysis for phospho-JNK (pJNK) and pJun in control and Bmp7 null cloacal tissues at E12.5. ß-actin was used as a loading control.