Domain Analysis Reveals That a Deubiquitinating Enzyme USP13 Performs Non-Activating Catalysis for Lys63-Linked Polyubiquitin
Figure 1
USP13 prefers to bind with Ub.
A, Domain architectures of USP5 and USP13. NT, N-terminal domain; ZnF, zinc-finger domain; UBA, Ub associated domain; C-box and H-box, two separated lobes of the catalytic domain. B, GST pull-down analysis of USP13 (purified with His6 tag from E.coli) with GST-fused Ub and other UbL proteins. 50% USP13 was loaded as a control. The samples were analyzed by SDS-PAGE with Coomassie blue staining.