The Inheritance of Histone Modifications Depends upon the Location in the Chromosome in Saccharomyces cerevisiae
Figure 2
Newly deposited nucleosomes and preexisting nucleosomes are not always deposited on the chromatin fiber in an alternating manner.
ChIP-on-chip analysis was conducted using mononucleosomes harboring the histone H3-G-Flag prepared previously (Figure 1E). Empty and filled circles represent the positions of newly deposited and preexisting nucleosomes, respectively. The map shows the 20 kbp region from 170 kbp to 190 kbp on the right arm of chromosome III. The horizontal lines indicate the log 1 of the signal strength, and the vertical scale bar indicates the chromosomal coordinates in kb. Blue horizontal lines indicate the open reading frames (ORFs); the ORFs above the horizontal line are oriented in the 5′ to 3′ direction from left to right, and the genes below are oriented in the reverse direction. A single bar of signal indicates 50 bp, and a set of three bars is approximately equivalent to one nucleosome (∼160 bp).