Runx1 Loss Minimally Impacts Long-Term Hematopoietic Stem Cells
Figure 7
Microarray analysis of genes mis-expressed in Runx1 deficient HSCs.
A. Heat plot of 4707 probe sets, representing 3820 genes, differentially expressed between LSKF- and LSKF+ cells isolated from mice transplanted with Runx1f/f and Runx1f/f; Mx1-Cre bone marrow, with a significance threshold of P = 0.005. Each column shows gene expression from donor cells from an independent transplant recipient. Red, > than the average, blue, < than the average. B. Quantitative real-time PCR (qRT-PCR) performed on several genes to independently validate the microarray data. Data shown are the mean fold change in expression (± SEM), normalized to Gapdh expression, between Runx1Δ/Δ (n = 3) and Runx1f/f (n = 3) LSKF- samples. C. GSEA profiles illustrating the correlation between genes negatively regulated by Runx1 in LSK cells, and genes sets of cell cycle and p53 pathways. Enrichment score, p values and FDR q-values are shown under the enrichment plots. Number of permutations was set to 1000. D. Venn diagram illustrating overlap between dysregulated genes in Runx1 deficient LSKF- and LSKF+ cells, and genes occupied by Runx1 in the HPC-7 line [44]. For list of genes see Table S4.