Cyclical and Patch-Like GDNF Distribution along the Basal Surface of Sertoli Cells in Mouse and Hamster Testes
Figure 8
GDNF-positive signals largely overlap with the areas of c-kit-positive spermatogonia in hamsters and mice.
Whole-mount immunostaining (without permeabilization) of seminiferous tubules showing GDNF-positive deposits (green) and c-kit-positive spermatogonia (red) in the basal surface of seminiferous epithelia in hamsters (A) and mice (B). Merged images (lower plates) visualize that the majority of GDNF deposits are overlapped with the areas of the c-kit-positive differentiated spermatogonia (arrowheads). Arrows indicate GDNF deposits that are seen in the c-kit-negative areas. Scale bar represents 100 µm.