Tumour Tissue Microenvironment Can Inhibit Dendritic Cell Maturation in Colorectal Cancer
Figure 1
Tumour Conditioned Media affects IL-10 and IL-12p70 secretion in a dose dependent manner.
Immature monocyte derived dendritic cells (iDCs) (n = 2) were treated for 4 hours with 1∶2, 1∶4 and 1∶10 dilutions of Tumour Conditioned Media (TCM) obtained by culturing the explanted tumours of 4 colorectal cancer patients in vitro for 72 hours. LPS (1 µg/mL) was added and the cells were cultured for a further 18 hours. Supernatants were collected from the cells cultured as described above, and the levels of IL-10 and IL-12p70 secretion by the DCs were measured by ELISA. Statistical differences were determined by Mann-Whitney U test.