BMI-1 Autoantibody as a New Potential Biomarker for Cervical Carcinoma
Figure 3
ELISA of phage-expressing BMI-1 proteins with individual serum samples.
The assays were performed with serially diluted (1∶20 to 1∶2560) individual serum samples that were not used in the biopan to confirm that measurements were representative of an antigen-antibody affinity reaction. Representative curves from four patients (P1, P2, P3, P4, P5) are shown for each protein. Measurements are expressed as mean absorbance minus the value of each serum sample assayed against empty T7 phage (background) 6 SEM absorbance. Data from empty T7 phage at each dilution, for each patient sample, are shown as a reference.