Darinaparsin Is a Multivalent Chemotherapeutic Which Induces Incomplete Stress Response with Disruption of Microtubules and Shh Signaling
Figure 1
Darinaparsin and sodium arsenite induce RNA stress granules.
HeLa cells were treated with 0.5 mM Sodium arsenite or 0.05 mM darinaparsin for 45 minutes at 37°C. Cells were fixed with supplemented 4% PFA. All cells are stained for DNA with Dapi (blue) and A. G3BP (green) and GW-182 (red), B. AKAP350 (green) and G3BP (red), C. Caprin-1 (green) and G3BP (red), D. CCAR1 (green) and G3BP (red). E. The number of granules in sodium arsenite- and darinaparsin-treated cells was quantified using ImageJ software. Results are mean +/− standard error. *p<0.0001 (Scale bars: 20 µM).