The Role of Proline Rich Tyrosine Kinase 2 (Pyk2) on Cisplatin Resistance in Hepatocellular Carcinoma
Figure 1
The establishment of Pyk2 overexpressed and suppressed stable transfectants.
(A), Western blot analysis verified the protein expression level of Pyk2 in different stable transfectants (PLC, Hep3B and MHCC97L). Significantly higher Pyk2 expression was observed in PLC-Pyk2-8 and Hep3B-Pyk2-11. Pyk2 expression level was knocked down in MHCC97L by transfecting C-terminal of Pyk2. (B), RT-PCR demonstrated Pyk2 expression level in different stable transfectants at mRNA level. (C), Real time RT-PCR quantified Pyk2 expression level in these stable transfectants. (D), MTT assay showed that overexpression of Pyk2 promoted HCC cell proliferation rates upon increasing concentrations of cisplatin treatment.