Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Probing the Limits of Aptamer Affinity with a Microfluidic SELEX Platform

Figure 3

Screening of clones for target binding.

We quantified the relative binding affinities of 20 clones from each selection using a bead-based fluorescence binding assay at a single ssDNA concentration. NB indicates a non-binding control sequence included in each measurement. We chose the top binding sequences for further analysis and determination of equilibrium dissociation binding constant (Kd). (A) PDGF-BB with [ssDNA] = 0.50 nM, NB = Thrombin Clone #1. (B) Thrombin with [ssDNA] = 0.50 nM, NB = PDGF Clone #1. (C) ApoE with [ssDNA] = 35 nM, NB = Thrombin Clone #1.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0027051.g003