Probing the Limits of Aptamer Affinity with a Microfluidic SELEX Platform
Figure 2
Affinities of round 3 pools for three target proteins.
Selections performed against three different proteins yielded enriched aptamer pools whose binding affinities were dependent on pI as determined by a bead-based fluorescence assay. (A) PDGF-BB round 3 pool Kd = 0.10 nM. (B) Thrombin round 3 pool Kd = 0.50 nM. (C) ApoE round 3 pool Kd = 9.3 nM.