LITAF Mediation of Increased TNF-α Secretion from Inflamed Colonic Lamina Propria Macrophages
Figure 2
Secreted TNF-α levels and LITAF message are increased in LPM harvested from inflamed colonic tissue compared to LPM harvested from non-inflamed colonic tissue.
A) Following TNBS administration, LPM were harvested and stimulated with LPS (1 ug/mL) ex vivo (24 hours) and TNF-α secretion was measured (N = 8). Data are presented as % Non-Inflamed Non-treated control ± SEM. * Statistical difference (p<0.05), # statistical difference (p<0.001). B) LITAF message levels are increased in LPM harvested from inflamed colonic tissue following LPS (1 ug/mL) stimulation (1 hour) compared to LPM harvested from non-inflamed colonic tissue (N = 7 for Non-treated, N = 8 LPS treated). Data were normalized to β-Actin message and presented as % Non-Inflamed Non-treated group ± SEM. * Statistical difference (p<0.05).