Induction of Eosinophil Apoptosis by the Cyclin-Dependent Kinase Inhibitor AT7519 Promotes the Resolution of Eosinophil-Dominant Allergic Inflammation
Figure 5
AT7519 effects on eosinophil apoptosis and subsequent clearance by macrophages is caspase dependent.
(A) Schematic representation of the experimental protocol. Immunized mice were challenged with OVA and 24 h later were treated with AT7519 and/or zVAD-fmk. The percentage of apoptotic eosinophils (B) and percentage of macrophages containing apoptotic bodies (C) was assessed 30 h after antigen-challenge. Results are expressed as the % cells per cavity, as a mean ± SEM of at least five mice in each group. ***P<0.001 when compared with vehicle-treated, OVA-injected mice. #P<0.05, ### P<0.001 when compared with AT7519-treated, OVA-injected mice.