The Breadth, but Not the Magnitude, of Circulating Memory B Cell Responses to P. falciparum Increases with Age/Exposure in an Area of Low Transmission
Figure 9
Atypical and classical memory B cells in children 2 months after a malaria episode.
AMB were defined as being CD19+CD21−CD27−CD10−, classical MBC were defined as CD19+CD21+CD27+ [21]. The gating strategy is shown in (A). Viable PBMC were gated using FSC/SSC (not shown) and then displayed according to CD19+ expression. The CD19+ population was displayed according to their expression of CD21 and CD27. To identify atypical MBCs, CD10− cells were subsequently selected from CD21−CD27− cells, using a CD10 gate defined on the entire PBMC population. (B) Percentages of CD19+ cells expressing the atypical and classical MBC phenotype are shown for children <5 and >5 years of age. P-values are given for the Mann-Whitney test.