A Time-Series Method for Automated Measurement of Changes in Mitotic and Interphase Duration from Time-Lapse Movies
Figure 1
‘Area’ and ‘Average Intensity’ features are sufficient to identify the boundaries of mitosis.
(a) A portion of an image of HeLa cells expressing the fluorescent marker H2B-GFP is shown. The red asterisk marks an example of a nucleus automatically tracked throughout a division. Images were acquired every 12 minutes. At the time of division (between frames 4 and 5) each daughter nucleus is tracked independently to produce a “trace” for the division. The images of the trace are then used for the time-series analysis as shown in (b). (b) An example of a trace, showing the values of average intensity (I) and area (A) graphed over time with montage of nucleus images. The interphase-prophase transition (IPT) and metaphase-anaphase transition (MAT) points are identified.