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HIF-1 and SKN-1 Coordinate the Transcriptional Response to Hydrogen Sulfide in Caenorhabditis elegans

Figure 1

Exposure to H2S induces rapid and progressive changes in mRNA abundance.

A. Experimental design schematic. C. elegans were grown from synchronized first-stage larvae (L1) for 48 h to young adult before being collected for RNA extraction. Each bar represents 48 h from L1 to first-day adult for one experimental group. Time in room air is indicated in white and time in H2S indicated in red. Exposure to H2S (50 ppm in room air) was always immediately prior to isolating RNA. B. Changes in mRNA abundance measured by microarray. Plots show magnitude of change in transcript level (log2 FC) as a function of adjusted p-value (log10 p-value). Each point is data from one gene product. Significant changes (adj. p-value<0.05) are red. After 1 h exposure to H2S (left), 16 genes were significantly up-regulated and one was down-regulated (Table 1). After 12 h exposure to H2S (middle), 445 transcripts were significantly changed, with 259 up-regulated (Tables 1 and S1). After 48 h in H2S (right), 5089 transcripts were significantly altered relative to untreated controls (Table S2).

Figure 1

doi: https://doi.org/10.1371/journal.pone.0025476.g001