Styryl-Based and Tricyclic Compounds as Potential Anti-Prion Agents
Figure 4
Structure and Anti-Prion Activity of Trimipramine.
Trimipramine. A) Shows the structure of trimipramine. Trimipramine was applied to N2a/22L for 72 hrs at 0.1, 0.5, 1.0, 2.5 and 5 µM. B) Western blot of PK-digested cell lysates. C) Bar graph representation of PrPSc reduction. Trimipramine reduces PrPSc infectivity by 89.6%, 77.7%, 68.2%, 22.7% and 5.6% respectively. Differences are significant by One-way ANOVA (***p<0.0001), and *p<0.05 Newman-Keuls post-hoc analysis versus N2a/22L as indicated on the graph.