Insights into the Role of Yeast eIF2A in IRES-Mediated Translation
Figure 3
Post-translationally modified eIF2A appears in 80S preinitiation complexes during 6% ethanol stress.
A, BY4684 ΔeIF2A yeast transformed with YCplac111-YP with or without 6% ethanol treatment were analyzed by polysome profiling. B, Subsequently, the top and 80S complex regions of the gradients (shown in A) were analyzed by 2D-gel electrophoresis and Western blotting with α-HA antibody to detect HA-eIF2A.