Targeted Disruption of TgPhIL1 in Toxoplasma gondii Results in Altered Parasite Morphology and Fitness
Figure 2
Parasites lacking TgPhIL1 are shorter and wider than parasites that express TgPhIL1.
The maximum length and width of individual wild-type (RH), RHΔTgPhIL1, and RHΔTgPhIL1/PhIL1-C5 parasites were measured in differential interference contrast images such as those shown. The experiment was done in triplicate, and numbers indicate the average measurements from 100 parasites/experiment, in µm, plus or minus standard error. The RHΔTgPhIL1 parasites are shorter and wider than either the wild-type or complemented clones (unpaired student's t-test, p<0.05; n.s. = not significant). Scale bars = 5 µm.