MicroRNA 144 Impairs Insulin Signaling by Inhibiting the Expression of Insulin Receptor Substrate 1 in Type 2 Diabetes Mellitus
Figure 11
Expression of IRS1 in HeLa/3T3L1 cells transfected with pre- or anti- miR-144.
A. Endogenous levels of miR-144 and IRS1 in HeLa/3T3L1 cells as delta threshold cycle (ΔCt) value with respect to 18S rRNA. Previous reports [42]–[44] have also confirmed that miR-144 and IRS1 are expressed in HeLa cells. B. Relative miR-144 expression in HeLa/3T3L1 cells transfected with either pre- or anti- miR-144 at a concentration of 30 nM. C. Relative IRS1 mRNA expression in HeLa/3T3L1 cells transfected with either pre- or anti- miR-144at a concentration of 30 nM. Data presented as mean ± SEM (n = 3). Fold change below one are expressed as the negative values. Statistically significant differences are tested at p<0.05 significance. *p<0.05, **p<0.01, ***p<0.001. D. IRS1 immunoreactives in HeLa cells transfected with either pre- or anti- miR-144. The cells were fixed and immunolabeled with IRS1 antibody (green) and nuclear stain DAPI (blue). Left panel: Non-transfected cells; Middle panel: Pre miR-144 transfected cells showed reduced fluorescence intensity; Right panel: Anti miR-144 transfected cells showed increased fluorescence intensity. The data presented here is a representative of three independent experiments.