Bacteria-Specific Neutrophil Dysfunction Associated with Interferon-Stimulated Gene Expression in the Acute Respiratory Distress Syndrome
Figure 6
Reciprocal modification of cell death in neutrophils from ARDS patients expressing ISG.
(A–C) The release of LDH to assess necrosis, and (D–F) percent of soluble histone-associated DNA fragments to assess apoptosis, from isolated neutrophils after 4 h treatment. (A) Spontaneous necrosis. (B) S. aureus-induced necrosis. (C) P. aeruginosa-induced necrosis. (D) Spontaneous apoptotic cell death (E) S. aureus-induced apoptotic cell death. (F) P. aeruginosa-induced apoptotic cell death. Scatter plot of values in neutrophils with normal (black) and high ISG expression (ISG; red); median values are indicated by the like-colored bars; normal ISG (n = 50–63), high ISG (n = 17–22). *, p<0.05, **, p<0.01.