Scalable Transcriptome Preparation for Massive Parallel Sequencing
Figure 1
A schematic view of the automated process.
Module 1: Isolation of poly-A containing mRNA from total RNA. Module 2: Fragmentation followed by precipitation on carboxylic acid coated beads (CA-purification). Module 3: cDNA synthesis of purified and fragmented mRNA. Module 4: End repair, 3′ adenylation, adaptor ligation and a CA-purification to remove fragments lower than 200 bp. The samples are then enriched by PCR before a final CA-purification.