Caffeine Consumption Prevents Diabetes-Induced Memory Impairment and Synaptotoxicity in the Hippocampus of NONcZNO10/LTJ Mice
Figure 5
Caffeine preventes astrocytosis caused by diabetes.
Panel A shows representative GFAP immunoreactivity photographs of CA1, CA3 and dentate gyrus (DG) showing that diabetic mice displayed increased number of GFAP-positive cells in the hippocampus, but not when consuming caffeine. This was quantitatively confirmed by Western blot (B). GFAP immunoreactivity was normalised to β-actin in the same membrane and calculated as percentage of control. Black, grey and striped bars represent diabetes (Diab), diabetes plus caffeine and caffeine (Caff) groups, respectively. Data in panels (A) and (B) are representative of 4–5 animals per group and in panel (C) the data are mean±SEM of n = 5–6 mice in each experimental group. **P<0.01 versus control.