Investigation of the Staphylococcus aureus GraSR Regulon Reveals Novel Links to Virulence, Stress Response and Cell Wall Signal Transduction Pathways
Figure 2
GraXSR do not control their own synthesis.
Expression of the graXRS operon was followed using a graX'-lacZ transcriptional fusion in S. aureus strains HG001, ST1036 (ΔgraRS) and ST1070 (ΔgraX). β-Galactosidase assays were performed as described in Materials and Methods and measured during mid-exponential growth at 37°C in TSB (grey bars) or after treatment with 200 µg ml−1 colistin for the HG001 strain (black bar). Means and standard deviations values are presented from at least three independent experiments.